Pauline Wang

Pauline Wang, PhD.
Senior Research Associate

Education:
Ph.D. State University of New York at Stony Brook
B.Sc. Rensselaer Polytechnic Institute

Contact information:
Office: ESC 4035
Lab: ESC 4038/4039
Lab phone: 416-946-7121
Email: pauline.wang@utoronto.ca


Publications:

Mycobiome Sequencing and Analysis Applied to Fungal Community Profiling of the Lower Respiratory Tract During Fungal Pathogenesis.
McTaggart, LR, Copeland, JK, Surendra, A, Wang, PW, Husain, S, Coburn, B, Guttman, DS, Kus, JV.
Front Microbiol.10,2019. PubMed PMID:30930884

Microbiome networks and change-point analysis reveal key community changes associated with cystic fibrosis pulmonary exacerbations.
Layeghifard, M, Li, H, Wang, PW, Donaldson, SL, Coburn, B, Clark, ST, Caballero, JD, Zhang, Y, Tullis, DE, Yau, YCW, Waters, V, Hwang, DM, Guttman, DS.
NPJ Biofilms Microbiomes.5,2019. PubMed PMID:30675371

Penicillin-binding protein 3 is a common adaptive target among Pseudomonas aeruginosa isolates from adult cystic fibrosis patients treated with β-lactams.
Clark, ST, Sinha, U, Zhang, Y, Wang, PW, Donaldson, SL, Coburn, B, Waters, VJ, Yau, YCW, Tullis, DE, Guttman, DS, Hwang, DM.
Int. J. Antimicrob. Agents.53,2019. PubMed PMID:30664925

Recombination of ecologically and evolutionarily significant loci maintains genetic cohesion in the Pseudomonas syringae species complex.
Dillon, MM, Thakur, S, Almeida, RND, Wang, PW, Weir, BS, Guttman, DS.
Genome Biol.20,2019. PubMed PMID:30606234

Identifying Pseudomonas syringae Type III Secreted Effector Function via a Yeast Genomic Screen.
Lee, AH, Bastedo, DP, Youn, JY, Lo, T, Middleton, MA, Kireeva, I, Lee, JY, Sharifpoor, S, Baryshnikova, A, Zhang, J, Wang, PW, Peisajovich, SG, Constanzo, M, Andrews, BJ, Boone, CM, Desveaux, D, Guttman, DS.
G3 (Bethesda).9,2019. PubMed PMID:30573466

Search PubMed

Popovic A, Bourdon C, Wang PW, Guttman DS, Voskuijl W, Grigg ME, Bandsma RHJ, Parkinson J. Design and application of a novel two-amplicon approach for defining eukaryotic microbiota. Microbiome. 2018 Dec 20;6(1):228. doi: 10.1186/s40168-018-0612-3. PubMed PMID: 30572961; PubMed Central PMCID: PMC6302507.

Diaz Caballero J, Clark ST, Wang PW, Donaldson SL, Coburn B, Tullis DE, Yau YCW, Waters VJ, Hwang DM, Guttman DS. A genome-wide association analysis reveals a potential role for recombination in the evolution of antimicrobial resistance in Burkholderia multivorans. PLoS Pathog. 2018 Dec;14(12):e1007453. doi: 10.1371/journal.ppat.1007453. eCollection 2018 Dec. PubMed PMID: 30532201; PubMed Central PMCID: PMC6300292.

Fitzpatrick CR, Lu-Irving P, Copeland J, Guttman DS, Wang PW, Baltrus DA, Dlugosch KM, Johnson MTJ. Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies. Microbiome. 2018 Aug 18;6(1):144. doi: 10.1186/s40168-018-0534-0. PubMed PMID: 30121081; PubMed Central PMCID: PMC6098832.

Middleton MA, Layeghifard M, Klingel M, Stanojevic S, Yau YCW, Zlosnik JEA, Coriati A, Ratjen FA, Tullis ED, Stephenson A, Wilcox P, Freitag A, Chilvers M, McKinney M, Lavoie A, Wang PW, Guttman DS, Waters VJ. Epidemiology of Clonal Pseudomonas aeruginosa Infection in a Canadian Cystic Fibrosis Population. Ann Am Thorac Soc.2018 Jul;15(7):827-836. doi: 10.1513/AnnalsATS.201801-007OC. PubMed PMID: 29911888.

Fitzpatrick CR, Copeland J, Wang PW, Guttman DS, Kotanen PM, Johnson MTJ.Assembly and ecological function of the root microbiome across angiosperm plant species. Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):E1157-E1165. doi: 10.1073/pnas.1717617115. Epub 2018 Jan 22. PubMed PMID: 29358405; PubMed Central PMCID: PMC5819437.


Beaudoin T, Yau YCW, Stapleton PJ, Gong Y, Wang PW, Guttman DS, Waters V.Staphylococcus aureus interaction with Pseudomonas aeruginosa biofilm enhances tobramycin resistance. NPJ Biofilms Microbiomes. 2017;3:25. doi: 10.1038/s41522-017-0035-0. eCollection 2017. PubMed PMID: 29062489; PubMed Central PMCID: PMC5648753.


Laflamme B, Middleton M, Lo T, Desveaux D, Guttman DS. Image-Based Quantification of Plant Immunity and Disease. Mol Plant Microbe Interact. 2016 Dec;29(12):919-924. doi: 10.1094/MPMI-07-16-0129-TA. Epub 2016 Dec 20. PubMed PMID: 27996374.

Mott GA, Thakur S, Smakowska E, Wang PW, Belkhadir Y, Desveaux D, Guttman DS.Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation.Genome Biol. 2016 May 9;17:98. doi: 10.1186/s13059-016-0955-7. PubMed PMID: 27160854; PubMed Central PMCID: PMC4862170.


Kim SH, Clark ST, Surendra A, Copeland JK, Wang PW, Ammar R, Collins C, Tullis DE, Nislow C, Hwang DM, Guttman DS, Cowen LE. Global Analysis of the Fungal Microbiome in Cystic Fibrosis Patients Reveals Loss of Function of the Transcriptional Repressor Nrg1 as a Mechanism of Pathogen Adaptation. PLoS Pathog. 2015 Nov;11(11):e1005308. doi: 10.1371/journal.ppat.1005308. eCollection 2015 Nov. PubMed PMID: 26588216; PubMed Central PMCID: PMC4654494.

Diaz Caballero J, Clark ST, Coburn B, Zhang Y, Wang PW, Donaldson SL, Tullis DE, Yau YC, Waters VJ, Hwang DM, Guttman DS. Selective Sweeps and Parallel Pathoadaptation Drive Pseudomonas aeruginosa Evolution in the Cystic Fibrosis Lung. MBio. 2015 Sep 1;6(5):e00981-15. doi: 10.1128/mBio.00981-15. PubMed PMID: 26330513; PubMed Central PMCID: PMC4556809.

Clark ST, Diaz Caballero J, Cheang M, Coburn B, Wang PW, Donaldson SL, Zhang Y, Liu M, Keshavjee S, Yau YC, Waters VJ, Elizabeth Tullis D, Guttman DS, Hwang DM.Phenotypic diversity within a Pseudomonas aeruginosa population infecting an adult with cystic fibrosis. Sci Rep. 2015 Jun 5;5:10932. doi: 10.1038/srep10932. PubMed PMID: 26047320; PubMed Central PMCID: PMC4456944.

Coburn B, Wang PW, Diaz Caballero J, Clark ST, Brahma V, Donaldson S, Zhang Y, Surendra A, Gong Y, Elizabeth Tullis D, Yau YC, Waters VJ, Hwang DM, Guttman DS.Lung microbiota across age and disease stage in cystic fibrosis. Sci Rep. 2015 May 14;5:10241. doi: 10.1038/srep10241. PubMed PMID: 25974282; PubMed Central PMCID: PMC4431465.

Copeland JK, Yuan L, Layeghifard M, Wang PW, Guttman DS. Seasonal community succession of the phyllosphere microbiome. Mol Plant Microbe Interact. 2015 Mar;28(3):274-85. doi: 10.1094/MPMI-10-14-0331-FI. PubMed PMID: 25679538.


Lewis JD, Lee AH, Hassan JA, Wan J, Hurley B, Jhingree JR, Wang PW, Lo T, Youn JY, Guttman DS, Desveaux D. The Arabidopsis ZED1 pseudokinase is required for ZAR1-mediated immunity induced by the Pseudomonas syringae type III effector HopZ1a.Proc Natl Acad Sci U S A. 2013 Nov 12;110(46):18722-7. doi: 10.1073/pnas.1315520110. Epub 2013 Oct 29. PubMed PMID: 24170858; PubMed Central PMCID: PMC3831984.

McCann HC, Rikkerink EH, Bertels F, Fiers M, Lu A, Rees-George J, Andersen MT, Gleave AP, Haubold B, Wohlers MW, Guttman DS, Wang PW, Straub C, Vanneste JL, Rainey PB, Templeton MD. Genomic analysis of the Kiwifruit pathogen Pseudomonas syringae pv. actinidiae provides insight into the origins of an emergent plant disease. PLoS Pathog.2013;9(7):e1003503. doi: 10.1371/journal.ppat.1003503. Epub 2013 Jul 25. PubMed PMID: 23935484; PubMed Central PMCID: PMC3723570.


Schreiber KJ, Austin RS, Gong Y, Zhang J, Fung P, Wang PW, Guttman DS, Desveaux D.Forward chemical genetic screens in Arabidopsis identify genes that influence sensitivity to the phytotoxic compound sulfamethoxazole. BMC Plant Biol. 2012 Nov 24;12:226. doi: 10.1186/1471-2229-12-226. PubMed PMID: 23176361; PubMed Central PMCID: PMC3541222.

Maughan H, Wang PW, Diaz Caballero J, Fung P, Gong Y, Donaldson SL, Yuan L, Keshavjee S, Zhang Y, Yau YC, Waters VJ, Tullis DE, Hwang DM, Guttman DS. Analysis of the cystic fibrosis lung microbiota via serial Illumina sequencing of bacterial 16S rRNA hypervariable regions. PLoS One. 2012;7(10):e45791. doi: 10.1371/journal.pone.0045791. Epub 2012 Oct 2. PubMed PMID: 23056217; PubMed Central PMCID: PMC3462755.

O’Brien HE, Thakur S, Gong Y, Fung P, Zhang J, Yuan L, Wang PW, Yong C, Scortichini M, Guttman DS. Extensive remodeling of the Pseudomonas syringae pv. avellanae type III secretome associated with two independent host shifts onto hazelnut. BMC Microbiol.2012 Jul 16;12:141. doi: 10.1186/1471-2180-12-141. PubMed PMID: 22800299; PubMed Central PMCID: PMC3411506.

Maughan H, Cunningham KS, Wang PW, Zhang Y, Cypel M, Chaparro C, Tullis DE, Waddell TK, Keshavjee S, Liu M, Guttman DS, Hwang DM. Pulmonary bacterial communities in surgically resected noncystic fibrosis bronchiectasis lungs are similar to those in cystic fibrosis. Pulm Med. 2012;2012:746358. doi: 10.1155/2012/746358. Epub 2012 Feb 8. PubMed PMID: 22448327; PubMed Central PMCID: PMC3289866.

Lee AH, Hurley B, Felsensteiner C, Yea C, Ckurshumova W, Bartetzko V, Wang PW, Quach V, Lewis JD, Liu YC, Börnke F, Angers S, Wilde A, Guttman DS, Desveaux D. A bacterial acetyltransferase destroys plant microtubule networks and blocks secretion.PLoS Pathog. 2012 Feb;8(2):e1002523. doi: 10.1371/journal.ppat.1002523. Epub 2012 Feb 2. PubMed PMID: 22319451; PubMed Central PMCID: PMC3271077.

Lewis JD, Wan J, Ford R, Gong Y, Fung P, Nahal H, Wang PW, Desveaux D, Guttman DS.Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2. BMC Genomics. 2012 Jan 9;13:8. doi: 10.1186/1471-2164-13-8. PubMed PMID: 22230763; PubMed Central PMCID: PMC3320541.


O’Brien HE, Gong Y, Fung P, Wang PW, Guttman DS. Use of low-coverage, large-insert, short-read data for rapid and accurate generation of enhanced-quality draft Pseudomonas genome sequences. PLoS One. 2011;6(11):e27199. doi: 10.1371/journal.pone.0027199. Epub 2011 Nov 2. PubMed PMID: 22073286; PubMed Central PMCID: PMC3206934.

Austin RS, Vidaurre D, Stamatiou G, Breit R, Provart NJ, Bonetta D, Zhang J, Fung P, Gong Y, Wang PW, McCourt P, Guttman DS. Next-generation mapping of Arabidopsis genes. Plant J. 2011 Aug;67(4):715-25. doi: 10.1111/j.1365-313X.2011.04619.x. Epub 2011 Jul 18. PubMed PMID: 21518053.


Pitout JD, Campbell L, Church DL, Wang PW, Guttman DS, Gregson DB. Using a commercial DiversiLab semiautomated repetitive sequence-based PCR typing technique for identification of Escherichia coli clone ST131 producing CTX-M-15. J Clin Microbiol. 2009 Apr;47(4):1212-5. doi: 10.1128/JCM.02265-08. Epub 2009 Feb 9. PubMed PMID: 19204095; PubMed Central PMCID: PMC2668328.


Wang PW, Morgan RL, Scortichini M, Guttman DS. Convergent evolution of phytopathogenic pseudomonads onto hazelnut. Microbiology. 2007 Jul;153(Pt 7):2067-73. doi: 10.1099/mic.0.2006/001545-0. PubMed PMID: 17600051.


Guttman DS, Gropp SJ, Morgan RL, Wang PW. Diversifying selection drives the evolution of the type III secretion system pilus of Pseudomonas syringae. Mol Biol Evol. 2006 Dec;23(12):2342-54. doi: 10.1093/molbev/msl103. Epub 2006 Sep 1. PubMed PMID: 16950758.


Hwang MS, Morgan RL, Sarkar SF, Wang PW, Guttman DS. Phylogenetic characterization of virulence and resistance phenotypes of Pseudomonas syringae.Appl Environ Microbiol. 2005 Sep;71(9):5182-91. doi: 10.1128/AEM.71.9.5182-5191.2005. PubMed PMID: 16151103; PubMed Central PMCID: PMC1214625.


Wang PW, Chu L, Guttman DS. Complete sequence and evolutionary genomic analysis of the Pseudomonas aeruginosa transposable bacteriophage D3112. J Bacteriol. 2004 Jan;186(2):400-10. doi: 10.1128/jb.186.2.400-410.2004. PubMed PMID: 14702309; PubMed Central PMCID: PMC305741.

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